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Article

Hemoglobin site-mutants reveal dynamical role of interhelical H-bonds in the allosteric pathway: time-resolved UV resonance Raman evidence for intra-dimer coupling.

Journal of molecular biology - 16 Jul 2004

Balakrishnan Gurusamy, Tsai Ching-Hsuan, Wu Qiang, Case Martin A, Pevsner Alex, McLendon George L, Ho Chien, Spiro Thomas G

Abstract excerpt

The dynamical effect of eliminating specific tertiary H-bonds in the hemoglobin (Hb) tetramer has been investigated by site-directed mutagenesis and time-resolved absorption and ultraviolet resonance Raman (UVRR) spectroscopy. The Trp alpha 14...Thr alpha 67 and Trp beta 15...Ser beta 72 H-bonds connect the A and E helices in the alpha and beta chains, and are proposed to break in the earliest protein...

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Identifiers

PMID: 15223326
DOI: 10.1016/j.jmb.2004.05.013

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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