Article
Identification of the structural gene for glucose-6-phosphate dehydrogenase in yeast. Inactivation leads to a nutritional requirement for organic sulfur.
The EMBO journal - 1 Mar 1991
Thomas D, Cherest H, Surdin-Kerjan Y
Abstract excerpt
Cloning of the MET19 gene revealed that it encodes the glucose-6-phosphate dehydrogenase from yeast. Sequence analysis showed a high degree of similarity between the yeast and the human enzymes. The cloned gene has allowed the construction of a glucose-6-phosphate dehydrogenase null mutant. The o...
Topics
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- Cysteine
- Escherichia coli
- Gene Expression Regulation, Fungal
- Genes, Fungal
- Genotype
- Glucosephosphate Dehydrogenase
- Glutathione
- Homocysteine
- Humans
- Kinetics
- Methionine
- Molecular Sequence Data
- Mutagenesis
- Phenotype
- Restriction Mapping