Article
The Afipia toolbox and its use to isolate flagellar mutants.
FEMS microbiology letters - 1 Jan 2010
Schueller Christian, Quinn Frederick D, Haas Albert
Abstract excerpt
Afipia felis, a Gram-negative alphaproteobacterium, has been implicated as one of the causative agents of cat scratch disease. To identify and begin to examine the virulence traits of this organism, we developed and tested a highly efficient transposon delivery system and a stable plasmid vector expressing green fluorescent protein. The transposome system is based on a Tn5-derived transposon and a phage...
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