Article
SIMPLE approach for isolating mutants expressing fimbriae.
Applied and environmental microbiology - 1 Jul 2007
Nuccio Sean-Paul, Chessa Daniela, Weening Eric H, Raffatellu Manuela, Clegg Steven, Bäumler Andreas J
Abstract excerpt
Genomes of members of the family Enterobacteriaceae contain large repertoires of putative fimbrial operons. Since many of these operons are poorly expressed in vitro, a convenient method for inducing elaboration of the encoded fimbriae would greatly facilitate their functional characterization. Here we describe a new technique for identifying fimbriated bacteria from a library of transposon mutants by screening...
Topics
- DNA Transposable Elements
- Fimbriae Proteins
- Fimbriae, Bacterial
- Immunomagnetic Separation
- Microscopy, Immunoelectron
- Mutagenesis, Insertional
- Mutation
- Salmonella typhimurium