Article
[Effects of SHIP gene mutation on cell cycle related proteins and phosphorylated Akt in K562 cells].
Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi - 1 Aug 2009
Yang Lin, Luo Jian-min, Liu Xiao-jun, Wen Shu-peng, Yang Jing-ci, Zhang Jing-yu
Abstract excerpt
OBJECTIVE: To investigate the effect of SHIP gene mutation on the cell cycle and its related gene expression in K562 cells. METHODS: The recombined green fluorescent protein (GFP) containing FIV-SHIP gene was transfected into K562 cells. The transfection efficiency and cell cycle of K562/SHIP were assessed by flow cytometry (FCM). The proliferation of K562 cells was detected by MTT assay, the mRNA levels of SHIP...
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