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Article

Optimization of allele-specific PCR using patient-specific HIV consensus sequences for primer design.

Journal of virological methods - 1 Mar 2010

Boltz Valerie F, Maldarelli Frank, Martinson Neil, Morris Lynn, McIntyre James A, Gray Glenda, Hopley Mark J, Kimura Toshio, Mayers Douglas L, Robinson Patrick, Mellors John W, Coffin John M, Palmer Sarah E

Abstract excerpt

Allele-specific PCR based on subtype consensus sequences is a powerful technique for detecting low frequency drug resistant mutants in HIV-1 infected patients. However, this approach can be limited by genetic variation in the region complementary to the primers, leading to variability in allele d...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 19948190
DOI: 10.1016/j.jviromet.2009.11.025
PMCID: PMC4734888

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-19948190-2026-09-02.2. Manifest publication-graph-cohort-2026-09-02.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

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