Article
Kinetic analysis of lactose exchange in proteoliposomes reconstituted with purified lac permease.
Biochemistry - 5 Feb 1991
Lolkema J S, Carrasco N, Kaback H R
Abstract excerpt
Lactose exchange catalyzed by purified lac permease reconstituted into proteoliposomes was analyzed with unequal concentrations of lactose on either side of the membrane and at low pH so as to prevent equilibration of the two pools. Exchange with external concentrations below 1.0 mM is a single-e...
Topics
- Biological Transport
- Escherichia coli
- Escherichia coli Proteins
- In Vitro Techniques
- Kinetics
- Lactose
- Membrane Transport Proteins
- Monosaccharide Transport Proteins
- Mutation
- Proteolipids
- Structure-Activity Relationship
- Symporters