Article
Unfolding and refolding properties of S pili on extraintestinal pathogenic Escherichia coli.
European biophysics journal : EBJ - 1 Jul 2010
Castelain Mickaël, Sjöström Annika E, Fällman Erik, Uhlin Bernt Eric, Andersson Magnus
Abstract excerpt
S pili are members of the chaperone-usher-pathway-assembled pili family that are predominantly associated with neonatal meningitis (S(II)) and believed to play a role in ascending urinary tract infections (S(I)). We used force-measuring optical tweezers to characterize the intrinsic biomechanical properties and kinetics of S(II) and S(I) pili. Under steady-state conditions, a sequential unfolding of the layers in...
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