Article
SNP discovery via 454 transcriptome sequencing.
The Plant journal : for cell and molecular biology - 1 Sept 2007
Barbazuk W Brad, Emrich Scott J, Chen Hsin D, Li Li, Schnable Patrick S
Abstract excerpt
A massively parallel pyro-sequencing technology commercialized by 454 Life Sciences Corporation was used to sequence the transcriptomes of shoot apical meristems isolated from two inbred lines of maize using laser capture microdissection (LCM). A computational pipeline that uses the POLYBAYES polymorphism detection system was adapted for 454 ESTs and used to detect SNPs (single nucleotide polymorphisms) between...
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