Article
SNP discovery by transcriptome pyrosequencing.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2011
Barbazuk W Brad, Schnable Patrick S
Abstract excerpt
Single nucleotide polymorphisms (SNPs) are single base differences between haplotypes. SNPs are abundant in many species and valuable as markers for genetic map construction, modern molecular breeding programs, and quantitative genetic studies. SNPs are readily mined from genomic DNA or cDNA sequence obtained from individuals having two or more distinct genotypes. While automated Sanger sequencing has become less...
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