Article
Chromogenic method for rapid isolation of recA-like mutants of gram-negative bacteria.
Journal of bacteriology - 1 Jan 1991
Barbe J, Fernandez de Henestrosa A R, Calero S, Gibert I
Abstract excerpt
We have devised a rapid and widely applicable color test for detecting recA-like mutants of gram-negative bacteria. The technique depends on decreased expression of an Escherichia coli recA-lacZ fusion in recA mutants and uses a broad-host-range plasmid to transfer the fusion gene into new species. We describe the isolation of a recA-like mutant of Pseudomonas syringae by this technique.
Topics
- Escherichia coli
- Gram-Negative Bacteria
- Kinetics
- Mutation
- Plasmids
- Pseudomonas
- Rec A Recombinases
- Recombinant Fusion Proteins
- Restriction Mapping
- Ultraviolet Rays