Article
New rapid and simple methods for detection of bacteria and determination of their antibiotic susceptibility by using phage mutants.
Applied and environmental microbiology - 1 Dec 2006
Ulitzur Nirit, Ulitzur Shimon
Abstract excerpt
Three new methods applying a novel approach for rapid and simple detection of specific bacteria, based on plaque formation as the end point of the phage lytic cycle, are described. Different procedures were designed to ensure that the resulting plaques were derived only from infected target bacteria ("infectious centers"). (i) A pair of amber mutants that cannot form plaques at concentrations lower than their...
Topics
- Anti-Bacterial Agents
- Bacteriological Techniques
- Bacteriolysis
- Bacteriophage Typing
- Bacteriophages
- Coliphages
- Enterobacteriaceae
- Escherichia coli
- Escherichia coli O157
- Lysogeny
- Microbial Sensitivity Tests