Article
Regulation of homotypic cell-cell adhesion by branched N-glycosylation of N-cadherin extracellular EC2 and EC3 domains.
The Journal of biological chemistry - 11 Dec 2009
Guo Hua-Bei, Johnson Heather, Randolph Matthew, Pierce Michael
Abstract excerpt
The effects of altering N-cadherin N-glycosylation on several cadherin-mediated cellular behaviors were investigated using small interfering RNA and site-directed mutagenesis. In HT1080 fibrosarcoma cells, small interfering RNA-directed knockdown of N-acetylglucosaminyltransferase V (GnT-V), a glycosyltransferase up-regulated by oncogene signaling, caused decreased expression of N-linked beta(1,6)-branched...
Topics
- Animals
- Cadherins
- Cell Adhesion
- Cell Line
- Cell Movement
- Enzyme Activation
- Glycoside Hydrolases
- Glycosylation
- Humans
- Mitogen-Activated Protein Kinases
- Mutation
- Polysaccharides
- Protein Structure, Tertiary
- Recombinant Fusion Proteins
- Signal Transduction