Article
Transformation of chloroplast ribosomal RNA genes in Chlamydomonas: molecular and genetic characterization of integration events.
Genetics - 1 Dec 1990
Newman S M, Boynton J E, Gillham N W, Randolph-Anderson B L, Johnson A M, Harris E H
Abstract excerpt
Transformation of chloroplast ribosomal RNA (rRNA) genes in Chlamydomonas has been achieved by the biolistic process using cloned chloroplast DNA fragments carrying mutations that confer antibiotic resistance. The sites of exchange employed during the integration of the donor DNA into the recipient genome have been localized using a combination of antibiotic resistance mutations in the 16S and 23S rRNA genes and...
Topics
- Chlamydomonas
- Chloroplasts
- Cloning, Molecular
- DNA, Ribosomal
- Drug Resistance
- Genes
- Mutation
- Phenotype
- Polymorphism, Restriction Fragment Length
- RNA, Ribosomal, 16S
- RNA, Ribosomal, 23S