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Article

Transformation and isolation of allelic exchange mutants of Chlamydia psittaci using recombinant DNA introduced by electroporation.

Proceedings of the National Academy of Sciences of the United States of America - 6 Jan 2009

Binet Rachel, Maurelli Anthony T

Abstract excerpt

To facilitate genetic investigations in the obligate intracellular pathogens Chlamydia, the ability to construct variants by homologous recombination was investigated in C. psittaci 6BC. The single rRNA operon was targeted with a synthetic 16S rRNA allele, harboring three nucleotide substitutions over 398 bp, which imparts resistance to kasugamycin (Ksm) and spectinomycin (Spc) and causes loss of one HpaI...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 19104068
DOI: 10.1073/pnas.0806768106
PMCID: PMC2629194

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Source receipt: pubmed, retrieved 7 Sept 2026.

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