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Article

High-efficiency biolistic transformation of Chlamydomonas mitochondria can be used to insert mutations in complex I genes.

Proceedings of the National Academy of Sciences of the United States of America - 21 Mar 2006

Remacle Claire, Cardol Pierre, Coosemans Nadine, Gaisne Mauricette, Bonnefoy Nathalie

Abstract excerpt

Mitochondrial transformation of Chlamydomonas reinhardtii has been optimized by using a particle-gun device and cloned mitochondrial DNA or PCR fragments. A respiratory-deficient strain lacking a 1.2-kb mitochondrial DNA region including the left telomere and part of the cob gene could be rescued as well as a double-frameshift mutant in the mitochondrial cox1 and nd1 genes. High transformation efficiency has been...

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Identifiers

PMID: 16537419
DOI: 10.1073/pnas.0509501103
PMCID: PMC1450245

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Source receipt: pubmed, retrieved 7 Sept 2026.

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