Article
High-efficiency biolistic transformation of Chlamydomonas mitochondria can be used to insert mutations in complex I genes.
Proceedings of the National Academy of Sciences of the United States of America - 21 Mar 2006
Remacle Claire, Cardol Pierre, Coosemans Nadine, Gaisne Mauricette, Bonnefoy Nathalie
Abstract excerpt
Mitochondrial transformation of Chlamydomonas reinhardtii has been optimized by using a particle-gun device and cloned mitochondrial DNA or PCR fragments. A respiratory-deficient strain lacking a 1.2-kb mitochondrial DNA region including the left telomere and part of the cob gene could be rescued as well as a double-frameshift mutant in the mitochondrial cox1 and nd1 genes. High transformation efficiency has been...
Topics
- Animals
- Biolistics
- Chlamydomonas
- DNA, Mitochondrial
- Drug Resistance
- Electron Transport Complex I
- Genes, Mitochondrial
- Genes, Protozoan
- Methacrylates
- Mitochondria
- Mutagenesis, Site-Directed
- Mutation
- Sequence Deletion