Article
Coating of nitrocellulose for colorimetric DNA microarrays.
Analytical biochemistry - 1 Feb 2010
Cretich Marina, Sedini Valentina, Damin Francesco, Pelliccia Maria, Sola Laura, Chiari Marcella
Abstract excerpt
We report on the modification of a nitrocellulose film with copoly(DMA-NAS-MAPS), a tercopolymer based on N,N-dimethylacrylamide (DMA), N-acryloyloxysuccinimide (NAS), and 3-(trimethoxysilyl)propyl-methacrylate (MAPS). The chains of this polymer, interacting with nitrocellulose fibers, introduce active ester functionalities that promote the covalent binding of short oligonucleotide fragments to the nitrocellulose...
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