Article
Detection of target DNA using fluorescent cationic polymer and peptide nucleic acid probes on solid support.
BMC biotechnology - 25 Apr 2005
Raymond Frédéric R, Ho Hoang-Anh, Peytavi Régis, Bissonnette Luc, Boissinot Maurice, Picard François J, Leclerc Mario, Bergeron Michel G
Abstract excerpt
BACKGROUND: Nucleic acids detection using microarrays requires labelling of target nucleic acids with fluorophores or other reporter molecules prior to hybridization. RESULTS: Using surface-bound peptide nucleic acids (PNA) probes and soluble fluorescent cationic polythiophenes, we show a simple and sensitive electrostatic approach to detect and identify unlabelled target nucleic acid on microarray. CONCLUSION:...
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