Article
Systematic analysis of interference due to stutter in estimating chimerism following hematopoietic cell transplantation.
Journal of clinical laboratory analysis - 1 Jan 2009
Thyagarajan Bharat, Young Shawn, Floodman Stacy, Peterson Randolph, Wang Xinjing
Abstract excerpt
Stutter formed during amplification of short tandem repeats (STRs) interfere with accurate engraftment monitoring following hematopoietic cell transplantation (HCT). We describe a mathematical approach to minimize the contribution of stutter when estimating chimerism following HCT. Pretransplant DNA samples from 409 donors and recipients were used to define marker-specific stutter cut-off values for all makers...
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