Article
Glutamine synthetase/glutamate synthase ammonium-assimilating pathway in Schizosaccharomyces pombe.
Current microbiology - 1 Jun 1995
Perysinakis A, Kinghorn J R, Drainas C
Abstract excerpt
Kinetic parameters of glutamine synthetase (GS) and glutamate synthase (glutamine-oxoglutarate aminotransferase) (GOGAT) activities, including initial velocity, pH, and temperature optima, as well as Km values, were estimated in Schizosaccharomyces pombe crude cell-free extracts. Five glutamine auxotrophic mutants of S. pombe were isolated following MNNG treatment. These were designated gln1-1,2,3,4,5, and their...
Topics
- Glutamate Synthase
- Glutamate-Ammonia Ligase
- Hydrogen-Ion Concentration
- Mutation
- Quaternary Ammonium Compounds
- Schizosaccharomyces
- Temperature