Article
Molecular characteristics of glnA linked mutations in the nitrogen-fixing cyanobacterium Nostoc muscorum.
Current microbiology - 1 Feb 2002
Srivastava Renu, Amla Devindra Vijay
Abstract excerpt
The glutamine synthetase (GS)-impaired mutants of Nostoc muscorum, isolated as ethylenediamine (EDA) resistant phenotype, were characterized for glnA linked processes to investigate the molecular mechanism of GS impairment. The GS enzyme was purified to homogeneity from the wild and mutant strains of N. muscorum employing ion-exchange followed by affinity chromatography. The final yield and specific activity of...
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