Role of NAD-linked glutamate dehydrogenase in nitrogen metabolism in Saccharomyces cerevisiae.

doi:10.1128/jb.172.9.4927-4935.1990

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Article

Role of NAD-linked glutamate dehydrogenase in nitrogen metabolism in Saccharomyces cerevisiae.

Journal of bacteriology - 1 Sept 1990

Miller S M, Magasanik B

Abstract excerpt

We cloned GDH2, the gene that encodes the NAD-linked glutamate dehydrogenase in the yeast Saccharomyces cerevisiae, by purifying the enzyme, making polyclonal antibodies to it, and using the antibodies to screen a lambda gt11 yeast genomic library. A yeast strain with a deletion-disruption allele...

Topics

Alleles
Ammonia
Cloning, Molecular
Escherichia coli
Genes, Fungal
Genetic Complementation Test
Genotype
Glutamate Dehydrogenase
Glutamate-Ammonia Ligase
Mutation
NAD
Nitrogen
Plasmids
Saccharomyces cerevisiae

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