Article
A NADP-glutamate dehydrogenase mutant of the petit-negative yeast Kluyveromyces lactis uses the glutamine synthetase-glutamate synthase pathway for glutamate biosynthesis.
Microbiology (Reading, England) - 1 Oct 1995
Valenzuela L, Guzmán-León S, Coria R, Ramírez J, Aranda C, González A
Abstract excerpt
The activities of the enzymes involved in ammonium assimilation and glutamate biosynthesis were determined in wild-type and NADP-glutamate dehydrogenase (GDH) null mutant strains of Kluyveromyces lactis. The specific NADP-GDH activity from K. lactis was fivefold lower than that found in Saccharom...
Topics
- Amino Acid Sequence
- Base Sequence
- Chromosome Mapping
- Genes, Bacterial
- Glutamate Dehydrogenase
- Glutamate Synthase
- Glutamate-Ammonia Ligase
- Glutamic Acid
- Kluyveromyces
- Molecular Sequence Data
- Mutation
