Article
Adenosylcobalamin-dependent methylmalonyl-CoA mutase from Propionibacterium shermanii. Active holoenzyme produced from Escherichia coli.
The Biochemical journal - 15 Jul 1990
McKie N, Keep N H, Patchett M L, Leadlay P F
Abstract excerpt
The linked structural genes coding for both subunits of adenosylcobalamin-dependent methylmalonyl-CoA mutase from the Gram-positive bacterium Propionibacterium shermanii have been altered by site-directed mutagenesis and placed under the control of an inducible phage-T7-specific plasmid promoter in Escherichia coli. Conditions have been found under which both alpha- and beta-subunits are produced in soluble form,...
Topics
- Base Sequence
- Cloning, Molecular
- Cobamides
- Crystallization
- Escherichia coli
- Gene Expression
- Genes, Bacterial
- Isomerases
- Methylmalonyl-CoA Mutase
- Molecular Sequence Data
- Mutation