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Article

The kinetic mechanism of wild-type and mutant mouse dihydrofolate reductases.

Biochemistry - 29 May 1990

Thillet J, Adams J A, Benkovic S J

Abstract excerpt

A kinetic mechanism is presented for mouse dihydrofolate reductase that predicts all the steady-state parameters and full time-course kinetics. This mechanism was derived from association and dissociation rate constants and pre-steady-state transients by using stopped-flow fluorescence and absorb...

Topics

Animals
Aspartic Acid
Binding Sites
Escherichia coli
Folic Acid
Glutamates
Glutamic Acid
Hydrogen-Ion Concentration
Kinetics
Mice
Mutation
Tetrahydrofolate Dehydrogenase

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 1974147
DOI: 10.1021/bi00473a028

Genes

DHFR

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-1974147-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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