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Article

The kinetic mechanism of wild-type and mutant mouse dihydrofolate reductases.

Biochemistry - 29 May 1990

Thillet J, Adams J A, Benkovic S J

Abstract excerpt

A kinetic mechanism is presented for mouse dihydrofolate reductase that predicts all the steady-state parameters and full time-course kinetics. This mechanism was derived from association and dissociation rate constants and pre-steady-state transients by using stopped-flow fluorescence and absorbance measurements. The major features of this kinetic mechanism are as follows: (1) the two native enzyme conformers,...

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Source

Identifiers

PMID: 1974147
DOI: 10.1021/bi00473a028

Genes

DHFR

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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