Article
The importance of loop region residues 40-46 in human dihydrofolate reductase as revealed by site-directed mutagenesis.
The Journal of biological chemistry - 15 May 1990
Tan X H, Huang S M, Ratnam M, Thompson P D, Freisheim J H
Abstract excerpt
Site-directed mutagenesis has been used to delete 2 residues (Gly45-Lys46) from a flexible "loop" region between residues 40 and 46 of human dihydrolate reductase. Steady-state kinetic studies show that the Km values for the deletion mutant enzyme for both dihydrofolate and nicotinamide adenine d...
Topics
- Antibodies
- Base Sequence
- Binding, Competitive
- Cloning, Molecular
- Cyanogen Bromide
- Enzyme Activation
- Enzyme-Linked Immunosorbent Assay
- Escherichia coli
- Folic Acid
- Humans
- Hydrogen-Ion Concentration
- Kinetics
- Methotrexate
- Molecular Sequence Data
- Mutation
- NADP
- Peptide Fragments
- Protein Conformation