Article
The importance of loop region residues 40-46 in human dihydrofolate reductase as revealed by site-directed mutagenesis.
The Journal of biological chemistry - 15 May 1990
Tan X H, Huang S M, Ratnam M, Thompson P D, Freisheim J H
Abstract excerpt
Site-directed mutagenesis has been used to delete 2 residues (Gly45-Lys46) from a flexible "loop" region between residues 40 and 46 of human dihydrolate reductase. Steady-state kinetic studies show that the Km values for the deletion mutant enzyme for both dihydrofolate and nicotinamide adenine dinucleotide phosphate (reduced) (NADPH) as well as the pH rate profile are virtually identical to that of the wild...
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