Article
Using the polymerase chain reaction to estimate mutation frequencies and rates in human cells.
Mutation research - 1 Sept 1992
Cortopassi G A, Arnheim N
Abstract excerpt
The Polymerase Chain Reaction (PCR) has had a significant impact on molecular studies of human mutagenesis, mainly in the acceleration of molecular characterisation of mutant genes in cells isolated by a phenotypic selection. PCR can also be used to study genetic alterations in cells which have not undergone phenotypic selection. By modifying the standard PCR parameters, the presence of mutations can be assayed...
Topics
- Animals
- Chromosome Aberrations
- Humans
- Male
- Models, Genetic
- Mutation
- Polymerase Chain Reaction
- Recombination, Genetic
- Risk Factors
- Spermatozoa