Article
[Reduction of the number of mutant copies of mitochondrial DNA in tissues of irradiated mice in the postradiation period].
Genetika - 1 Jul 2009
Guliaeva N A, Abdullaev S A, Malakhova L V, Antipova V N, Bezlepkin V G, Gaziev A I
Abstract excerpt
Changes in the number of mutant copies of mitochondrial DNA (mtDNA) were studied in the brain and spleen tissues of mice after their X-irradiation at a dose of 5 Gy. For this purpose, heteroduplexes obtained via hybridization of the products of PCR amplification of mtDNA (ND3 gene and two D-loop regions) from irradiated and control mice were digested with the CelI nuclease capable of specific mismatch cleavage....
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