Article
Role of lysine-67 in the active site of class C beta-lactamase from Citrobacter freundii GN346.
European journal of biochemistry - 22 Feb 1990
Tsukamoto K, Tachibana K, Yamazaki N, Ishii Y, Ujiie K, Nishida N, Sawai T
Abstract excerpt
Citrobacter freundii GN346 produces a class C beta-lactamase exhibiting the substrate profile of a typical cephalosporinase. The structural and promoter regions of the cephalosporinase gene, comprising 1408 nucleotides, were completely sequenced. The amino acid sequence of the mature enzyme, comp...
Topics
- Amino Acid Sequence
- Arginine
- Base Sequence
- Binding Sites
- Cephalosporinase
- Citrobacter
- Escherichia coli
- Genes, Bacterial
- Glutamates
- Glutamic Acid
- Kinetics
- Lysine
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Promoter Regions, Genetic
- Protein Engineering
- RNA, Messenger