Article
Cloning and sequencing of the gene for the lactose carrier of Citrobacter freundii.
Biochemical and biophysical research communications - 30 Sept 1994
Lee J I, Okazaki N, Tsuchiya T, Wilson T H
Abstract excerpt
The gene coding for the lactose carrier of Citrobacter freundii was cloned into the plasmid pBR322. The gene was sequenced and the amino acid sequence was found to be 70% identical to the lactose carrier of E. coli. All of the charged residues in the membrane spanning region were conserved. The s...
Topics
- Amino Acid Sequence
- Base Sequence
- Citrobacter freundii
- Cloning, Molecular
- Escherichia coli
- Escherichia coli Proteins
- Genes, Bacterial
- Genomic Library
- Genotype
- Klebsiella
- Membrane Transport Proteins
- Molecular Sequence Data
- Monosaccharide Transport Proteins
- Nitrophenylgalactosides
- Plasmids
- Sequence Homology, Amino Acid
- Symporters