Article
[Minimal functional domain of cytidine 5'-monophosphate N-acetylneuraminic acid (CMP-NeuAc) synthetase from Escherichia coli].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology - 1 Nov 2002
Jin Chun-Sheng, Jin Cheng
Abstract excerpt
In comparison with its counterpart from N. meningitides, all conserved motifs were found in the N-termini of E. coli CMP-NeuAc synthetase. E. coli CMP-NeuAc synthetase seems to have redundant C-termini with a less effect on its activity. To explain this speculation, a series of recombinant DNAs with deletion from 3'-end of CMP-NeuAc synthetase were produced by PCR, ligated into expression vector pET-15b and...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
