Article
Identification of a catalytic residue of Clostridium paraputrificum N-acetyl-beta-D-glucosaminidase Nag3A by site-directed mutagenesis.
Bioscience, biotechnology, and biochemistry - 1 May 2006
Li Huazhong, Zhao Guangshan, Miyake Hideo, Umekawa Hayato, Kimura Tetsuya, Ohmiya Kunio, Sakka Kazuo
Abstract excerpt
Clostridium paraputrificum M-21 beta-N-acetylglucosaminidase 3A (Nag3A) is an enzyme classified in family 3 of the glycoside hydrolases. To identify catalytic residues of this enzyme, mutations were introduced into highly conserved Glu and Asp residues. Replacement of Asp175 with Ala abolished the catalytic activity without change in the circular dichroism spectrum, strongly suggesting that this residue is a...
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