Article
Fast-growing, non-infectious and intracellularly surviving drug-resistant Mycobacterium aurum: a model for high-throughput antituberculosis drug screening.
The Journal of antimicrobial chemotherapy - 1 Oct 2009
Gupta Antima, Bhakta Sanjib, Kundu Subir, Gupta Manish, Srivastava Brahm S, Srivastava Ranjana
Abstract excerpt
OBJECTIVES: Enoyl acyl-carrier-protein reductase (InhA), the primary endogenous target for isoniazid and ethionamide, is crucial to type-II fatty acid biosynthesis (FAS-II). The objectives of this study were first to generate InhA mutants of Mycobacterium aurum, secondly to characterize InhA-mediated isoniazid and ethionamide resistance mechanisms across those mutants and finally to investigate the interaction of...
Topics
- Antitubercular Agents
- Bacterial Proteins
- Circular Dichroism
- Colony Count, Microbial
- Drug Evaluation, Preclinical
- Drug Resistance, Bacterial
- Ethionamide
- Fatty Acids
- Gene Expression Profiling
- Isoniazid
- Microbial Viability
- Mutation