Article
LRRK2 regulates autophagic activity and localizes to specific membrane microdomains in a novel human genomic reporter cellular model.
Human molecular genetics - 1 Nov 2009
Alegre-Abarrategui Javier, Christian Helen, Lufino Michele M P, Mutihac Ruxandra, Venda Lara Lourenço, Ansorge Olaf, Wade-Martins Richard
Abstract excerpt
Leucine rich repeat kinase 2 (LRRK2) mutations are the most common genetic cause of Parkinson's disease (PD) although LRRK2 function remains unclear. We report a new role for LRRK2 in regulating autophagy and describe the recruitment of LRRK2 to the endosomal-autophagic pathway and specific membr...
Topics
- Adaptor Proteins, Signal Transducing
- Autophagy
- Brain
- Caveolae
- Cell Line
- Endosomes
- Green Fluorescent Proteins
- Humans
- Immunoblotting
- Immunoprecipitation
- Leucine-Rich Repeat Serine-Threonine Protein Kinase-2
- Membrane Microdomains
- Microscopy, Immunoelectron