Article
Leucyl-tRNA synthetase-dependent and -independent activation of a group I intron.
The Journal of biological chemistry - 25 Sept 2009
Boniecki Michal T, Rho Seung Bae, Tukalo Mikhail, Hsu Jennifer L, Romero Eliana P, Martinis Susan A
Abstract excerpt
Leucyl-tRNA synthetase (LeuRS) is an essential RNA splicing factor for yeast mitochondrial introns. Intracellular experiments have suggested that it works in collaboration with a maturase that is encoded within the bI4 intron. RNA deletion mutants of the large bI4 intron were constructed to identify a competently folded intron for biochemical analysis. The minimized bI4 intron was active in RNA splicing and...
Topics
- Base Sequence
- Binding Sites
- DNA, Fungal
- Electrophoresis, Polyacrylamide Gel
- Endoribonucleases
- Guanosine
- Introns
- Kinetics
- Leucine-tRNA Ligase
- Magnesium
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Nucleotidyltransferases