Article
An inserted region of leucyl-tRNA synthetase plays a critical role in group I intron splicing.
The EMBO journal - 16 Dec 2002
Rho Seung Bae, Lincecum Tommie L, Martinis Susan A
Abstract excerpt
Yeast mitochondrial leucyl-tRNA synthetase (LeuRS) binds to the bI4 intron and collaborates with the bI4 maturase to aid excision of the group I intron. Deletion analysis isolated the inserted LeuRS CP1 domain as a critical factor in the protein's splicing activity. Protein fragments comprised of just the LeuRS CP1 region rescued complementation of a yeast strain that expressed a splicing-defective LeuRS....
Topics
- Blotting, Western
- Escherichia coli
- Gene Deletion
- Introns
- Leucine-tRNA Ligase
- Mitochondria
- Models, Genetic
- Mutation
- Plasmids
- Protein Binding
- Protein Structure, Tertiary
- RNA
- RNA Splicing
- Reverse Transcriptase Polymerase Chain Reaction
- Two-Hybrid System Techniques