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Article

Determination of dissociation constants in living zebrafish embryos with single wavelength fluorescence cross-correlation spectroscopy.

Biophysical journal - 22 Jul 2009

Shi Xianke, Foo Yong Hwee, Sudhaharan Thankiah, Chong Shang-Wei, Korzh Vladimir, Ahmed Sohail, Wohland Thorsten

Abstract excerpt

The quantification of biological interactions is very important in life sciences. Here we report for the first time, to our knowledge, the determination of a biomolecular dissociation constant (K(D)) in living zebrafish embryos at physiological protein expression levels. For that purpose, we extend the application of single wavelength fluorescence cross-correlation spectroscopy into small organisms and measure...

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Identifiers

PMID: 19619483
DOI: 10.1016/j.bpj.2009.05.006
PMCID: PMC2711317

Genes

IQGAP1

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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