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Article

Simplified amplified-fragment length polymorphism method for genotyping Mycobacterium tuberculosis isolates.

Journal of microbiological methods - 1 Sept 2009

Viader-Salvadó José M, Flores-Gracia Juan, Vega-Alonso Agustina S, Treviño-Alvarado Víctor M, Molina-Torres Carmen A, Vera-Cabrera Lucio, Guerrero-Olazarán Martha

Abstract excerpt

A simplified amplified-fragment length polymorphism (AFLP) method was developed and applied to genotype 52 Mycobacterium tuberculosis isolates. This method can be carried out using only one restriction enzyme (XhoI), one double strand adapter, and one PCR primer. The amounts of DNA and DNA polymerase, and the concentrations of primer and Mg(2+) in the PCR step were optimized using the Basic Sequential Simplex...

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PMID: 19616590
DOI: 10.1016/j.mimet.2009.07.007

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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