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Routine use of a simple low-cost genotypic assay for the identification of mycobacteria in a high throughput laboratory.

Diagnostic microbiology and infectious disease - 1 Oct 2003

Wong Derek A, Yip Peter C W, Tse Denise L W, Tung Viola W N, Cheung Danny T L, Kam Kai Man

Abstract excerpt

A novel polymerase chain reaction (PCR)-restriction fragment length polymorphism analysis (PRA) of the hsp65 gene was used for the routine identification of mycobacteria in a high throughput clinical laboratory. A total of 2036 clinical isolates were tested by PRA in conjunction with other methods. The PRA identification of M. tuberculosis complex was 100% sensitive and specific, and 74.5% of nontuberculous...

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PMID: 14522516
DOI: 10.1016/s0732-8893(03)00133-0

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NTM

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

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