Article
Mg2+-assisted catalysis by B. stearothermophilus TrpRS is promoted by allosteric effects.
Structure (London, England : 1993) - 15 Jul 2009
Weinreb Violetta, Li Li, Campbell Cassandra L, Kaguni Laurie S, Carter Charles W
Abstract excerpt
Mn(2+)-assisted catalysis by B. stearothermophilus TrpRS parallels that in polymerases and reduces specificity in amino acid activation. As predicted by nonequilibrium molecular dynamics simulations, multivariant thermodynamic cycles with [ATP]-dependent Michaelis-Menten kinetics and Mn(2+) for Mg(2+) substitution demonstrate energetic coupling of ATP affinities to the metal; to lysines K111 and K192, which...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.