Article
A Cds1-mediated checkpoint protects the MBF activator Rep2 from ubiquitination by anaphase-promoting complex/cyclosome-Ste9 at S-phase arrest in fission yeast.
Molecular and cellular biology - 1 Sept 2009
Chu Zhaoqing, Eshaghi Majid, Poon Suk Yean, Liu Jianhua
Abstract excerpt
Transcription of the MluI cell cycle box (MCB) motif-containing genes at G(1) phase is regulated by the MCB-binding factors (MBF) (also called DSC1) in Schizosaccharomyces pombe. Upon S-phase arrest, the MBF transcriptional activity is induced through the accumulation of the MBF activator Rep2. In this study, we show that the turnover of Rep2 is attributable to ubiquitin-mediated proteolysis. Levels of Rep2...
Topics
- Amino Acid Sequence
- Anaphase-Promoting Complex-Cyclosome
- Cell Cycle Proteins
- Checkpoint Kinase 2
- DNA-Binding Proteins
- G1 Phase
- Hydroxyurea
- Mutation
- Phosphorylation
- Proteasome Endopeptidase Complex