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Article

NMR study of complexes between low molecular mass inhibitors and the West Nile virus NS2B-NS3 protease.

The FEBS journal - 1 Aug 2009

Su Xun-Cheng, Ozawa Kiyoshi, Yagi Hiromasa, Lim Siew P, Wen Daying, Ekonomiuk Dariusz, Huang Danzhi, Keller Thomas H, Sonntag Sebastian, Caflisch Amedeo, Vasudevan Subhash G, Otting Gottfried

Abstract excerpt

The two-component NS2B-NS3 protease of West Nile virus is essential for its replication and presents an attractive target for drug development. Here, we describe protocols for the high-yield expression of stable isotope-labelled samples in vivo and in vitro. We also describe the use of NMR spectroscopy to determine the binding mode of new low molecular mass inhibitors of the West Nile virus NS2B-NS3 protease...

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Source

Identifiers

PMID: 19583774
DOI: 10.1111/j.1742-4658.2009.07132.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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