Article
Dihydrofolate reductase gene amplification-associated shift of differentiation in methotrexate-adapted HT-29 cells.
The Journal of cell biology - 1 Dec 1991
Lesuffleur T, Barbat A, Luccioni C, Beaumatin J, Clair M, Kornowski A, Dussaulx E, Dutrillaux B, Zweibaum A
Abstract excerpt
Postconfluent cultures of HT-29 cells form a heterogeneous multilayer of which greater than 95% of the cells are undifferentiated. In contrast, when stably adapted to normally lethal concentrations of methotrexate (10(-6)-10(-5) M), they form a monolayer of gobletlike cells (Lesuffleur et al., 1990) which secrete large quantities of mucins and display a discrete brush border with the presence of villin,...
Topics
- Blotting, Southern
- Cell Differentiation
- Cell Division
- Cell Line
- Fluorescent Antibody Technique
- Gene Amplification
- Kinetics
- Methotrexate
- Microscopy, Electron
- Phenotype
- Tetrahydrofolate Dehydrogenase