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Article

Protein interaction platforms: visualization of interacting proteins in yeast.

Nature methods - 1 Jul 2009

Schmitz Alexa M, Morrison Monica F, Agunwamba Akochi O, Nibert Max L, Lesser Cammie F

Abstract excerpt

Here we describe the protein interaction platform assay, a method for identifying interacting proteins in Saccharomyces cerevisiae. This assay relies on the reovirus scaffolding protein microNS, which forms large focal inclusions in living cells. When a query protein is fused to microNS and potential interaction partners are fused to a fluorescent reporter, interactors can be identified by screening for yeast...

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Identifiers

PMID: 19483691
DOI: 10.1038/nmeth.1337
PMCID: PMC2819136

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

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