Article
A charged residue at the subunit interface of PCNA promotes trimer formation by destabilizing alternate subunit interactions.
Acta crystallographica. Section D, Biological crystallography - 1 Jun 2009
Freudenthal Bret D, Gakhar Lokesh, Ramaswamy S, Washington M Todd
Abstract excerpt
Eukaryotic proliferating cell nuclear antigen (PCNA) is an essential replication accessory factor that interacts with a variety of proteins involved in DNA replication and repair. Each monomer of PCNA has an N-terminal domain A and a C-terminal domain B. In the structure of the wild-type PCNA protein, domain A of one monomer interacts with domain B of a neighboring monomer to form a ring-shaped trimer. Glu113 is...
Topics
- Binding Sites
- Cloning, Molecular
- Crystallization
- Crystallography, X-Ray
- DNA Repair
- DNA Replication
- Glutamine
- Hydrophobic and Hydrophilic Interactions
- Models, Chemical
- Mutant Proteins
- Mutation
- Proliferating Cell Nuclear Antigen
- Protein Binding