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Article

A sensitive direct sequencing assay based on nested PCR for the detection of HBV polymerase and surface glycoprotein mutations.

Journal of virological methods - 1 Jul 2009

Vincenti Donatella, Solmone Mariacarmela, Garbuglia Anna Rosa, Iacomi Fabio, Capobianchi Maria Rosaria

Abstract excerpt

Drug resistance is a crucial problem emerging frequently during treatment of hepatitis B, resulting in treatment failure and progression of liver damage. A direct sequencing method based on a nested PCR was established to detect mutations in samples with low viral load. Primers were designed to o...

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Identifiers

PMID: 19442845
DOI: 10.1016/j.jviromet.2009.02.027

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-19442845-2026-09-02.2. Manifest publication-graph-cohort-2026-09-02.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

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