Rapid gynogenetic mapping of Xenopus tropicalis mutations to chromosomes.

doi:10.1002/dvdy.21965

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Article

Rapid gynogenetic mapping of Xenopus tropicalis mutations to chromosomes.

Developmental dynamics : an official publication of the American Association of Anatomists - 1 Jun 2009

Khokha Mustafa K, Krylov Vladimir, Reilly Michael J, Gall Joseph G, Bhattacharya Dipankan, Cheung Chung Yan J, Kaufman Sarah, Lam Dang Khoa, Macha Jaroslav, Ngo Catherine, Prakash Neha, Schmidt Philip, Tlapakova Tereza, Trivedi Toral, Tumova Lucie, Abu-Daya Anita, Geach Timothy, Vendrell Elisenda, Ironfield Holly, Sinzelle Ludivine, Sater Amy K, Wells Dan E, Harland Richard M, Zimmerman Lyle B

Abstract excerpt

Pilot forward genetic screens in Xenopus tropicalis have isolated over 60 recessive mutations. Here we present a simple method for mapping mutations to chromosomes using gynogenesis and centromeric markers. When coupled with available genomic resources, gross mapping facilitates evaluation of candidate genes as well as higher resolution linkage studies. Using gynogenesis, we have mapped the genetic locations of...

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PMID: 19441086
DOI: 10.1002/dvdy.21965
PMCID: PMC2962985

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