Article
Quantitative genotyping of mouse brain-specific PEX13 gene disruption by real-time PCR.
Journal of neuroscience methods - 30 Jun 2009
Müller C Catharina, Nourse Jamie P, Nguyen Tam H, Crane Denis I
Abstract excerpt
The Cre/loxP-system has become an invaluable tool for the generation of tissue-specific gene disruption in mice. However, because Cre recombinase excision of individual genes can be variable, an accurate and sensitive method is necessary to determine the ultimate level of gene disruption. The analysis of gene disruption is particularly difficult for tissue that has been fixed for (immuno)histochemical analysis...
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