Article
One-step cloning system for isolation of bacterial lexA-like genes.
Journal of bacteriology - 1 Nov 1991
Calero S, Garriga X, Barbé J
Abstract excerpt
A system to isolate lexA-like genes of bacteria directly was developed. It is based upon the fact that the presence of a lexA(Def) mutation is lethal to SulA+ cells of Escherichia coli. This system is composed of a SulA- LexA(Def) HsdR- strain and a lexA-conditional killer vector (plasmid pUA165)...
Topics
- Bacterial Proteins
- Cloning, Molecular
- DNA, Bacterial
- Erwinia
- Escherichia coli
- Genes, Bacterial
- Genes, Lethal
- Mutation
- Plasmids
- Pseudomonas aeruginosa
- Pseudomonas putida
- Recombinant Proteins
- Restriction Mapping
- Salmonella typhimurium
- Serine Endopeptidases
- Ultraviolet Rays
- beta-Galactosidase