Article
[New method of "ingredient difference phonetypical cloning" for functional gene cloning from medicinal plants].
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica - 1 Jan 2009
Wang Xueyong, Cui Guanghong, Gao Wei, Huang Luqi
Abstract excerpt
This paper introduced a new method of "ingredient difference phonetypical cloning" for functional gene clone of medicinal plants, which might solve the difficulties in isolating genes encoding enzymes for the biosynthesis of secondary metabolites by usual ways. Concepts, mechanisms and methods were systematically introduced and possibility was proved by experiments. The method showed the extra superiority of for...
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